<?xml version="1.0" encoding="UTF-8"?>
<archive_sequence>
  <submitter>sebastien.carrere@inrae.fr</submitter>
  <contributors>
    <contributor>Nicolas, Pouilly</contributor>
    <contributor>Nicolas, Langlade</contributor>
  </contributors>
  <date>20240111</date>
  <date_update>20240111</date_update>
  <sample>
    <title>E1264-ANN1430-654</title>
    <organisms>
      <organism>
        <species>Helianthus annuus</species>
        <genotype>PI468576</genotype>
        <taxid>4232</taxid>
      </organism>
    </organisms>
    <molecule>genomic_DNA</molecule>
    <growth_protocol>4-week-old seedlings of sunflower under greenhouse conditions, followed by 48 hours in the dark</growth_protocol>
    <extract_protocol>HMW gDNA extraction (Mayjonade, 2016)</extract_protocol>
  </sample>
  <sequencing>
    <protocol>
      <library_strategy>OTHER</library_strategy>
      <library_construction_protocol>Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0. Procedure and checklist, 102-166-600 REV02 MAR2023</library_construction_protocol>
      <library_construction_kit>SMRTBell prep kit 3.0 PacBio</library_construction_kit>
      <library_sequencing_kit>Revio sequencing plate et Revio SMRTCell tray</library_sequencing_kit>
      <library>
        <type>smrt-genomic</type>
      </library>
    </protocol>
    <platform>
      <seqcenter>Gentyane</seqcenter>
      <instrument_model>Revio</instrument_model>
    </platform>
    <collection>
      <repeat>none</repeat>
      <format>Revio_BAM</format>
      <left_adapter>CAACAACAACAACGGAGGAGGAGGAAAAG</left_adapter>
      <right_adapter>CAACAACAACAACGGAGGAGGAGGAAAAG</right_adapter>
      <files>
        <name>E1264-ANN1430-654</name>
        <file>
          <extension>bam</extension>
          <id>1</id>
          <internal>fc03dda1574c92f6b28f7c3a26f25784_E1264-ANN1430-654.m84087_231212_190002_s2.hifi_reads.bam</internal>
          <internal_id>45614</internal_id>
          <external>E1264-ANN1430-654.m84087_231212_190002_s2.hifi_reads.bam</external>
          <internal_path>/INRA-Toulouse/sequence/2024/01/11</internal_path>
          <readlen>0</readlen>
          <checksum>fc03dda1574c92f6b28f7c3a26f25784</checksum>
        </file>
        <file>
          <extension>xml</extension>
          <id>2</id>
          <internal>12383b15f17284849ecfd412dee7bfd1_E1264-ANN1430-654.m84087_231212_190002_s2.hifi_reads.consensusreadset.xml</internal>
          <internal_id>45613</internal_id>
          <external>E1264-ANN1430-654.m84087_231212_190002_s2.hifi_reads.consensusreadset.xml</external>
          <internal_path>/INRA-Toulouse/sequence/2024/01/11</internal_path>
          <readlen>0</readlen>
          <checksum>12383b15f17284849ecfd412dee7bfd1</checksum>
        </file>
      </files>
    </collection>
  </sequencing>
  <funding>
    <source>
      <project>HelEx</project>
      <institution>European Union</institution>
    </source>
  </funding>
</archive_sequence>
